School of Pharmacy UNIVERSITY OF WISCONSIN - MADISON NMR Spectroscopy Training NMR Training Overview Facility access is earned in stages. Completing the first stage of training earns basic access to the NMR Facility, comprising physical access to the laboratory and access to the Bruker 400 MHz spectrometer, data stations, printer, etc. Access to the Varian 500 MHz instrument requires completion of a second training component. Additional training is required for those desiring to perform variable-temperature experiments or other experiments of a more advanced nature. Those with minimal NMR knowledge and/or experience are expected to put forth extra effort as needed to get “up to speed” for independent work in the Facility; such efforts may include additional reading, study and hands-on practice. The NMR Facility Director will strive to provide training and direction as appropriate to meet individual needs. Commonly requested training sessions are described below, with links to related Facility documents and an online NMR Training Registration Form which must be used to request the training of interest. Arrangements for NMR training should be planned and made in advance to avoid scheduling conflicts, to understand the requirements and to allow adequate time to prepare for and complete the requirements. Basic Facility Access (Bruker AV-400, 400 MHz Spectrometer) Complete the online NMR Training Registration Form and request Basic Access training. You will be provided with a directed reading assignment from the NMR Facility User Guide. Once the reading assignment has been completed, you will answer a quiz over policies and other relevant topics from the assigned reading. When the quiz has been successfully completed, you will be contacted to schedule a training session. The training demonstration session requires a commitment of about 3 hours and covers basic spectrometer operation, plus an introduction to file handling, data processing options and the on-line instrument reservation utility. At the end of the training session, you will complete a practical exercise to acquire NMR data (a reproduction of the procedures covered in the training demonstration). Notes: Bear these time commitments in mind when scheduling the training session; you will need to schedule approximately 3-4 hours. If you anticipate difficulty in meeting these requirements, please discuss your concerns with the NMR Facility Director. An NMR sample will be provided for the demonstration and exercise. Although written documentation is available from a variety of sources, training session attendees are expected to take adequate notes during all demonstrations. Advanced Access (Varian UI-500, 500 MHz Spectrometer) Those with needs that cannot be met by the AV-400 spectrometer may request advanced training for access to the UI-500 spectrometer. Complete the online NMR Training Registration Form and request Advanced Access training. You will be provided with an appropriately directed reading assignment from the NMR Facility User Guide, along with a quiz over the reading material. Once the successfully completed quiz has been received by the NMR Facility Director, you will be contacted to schedule a training session. The training session requires a commitment of 2 hours and covers more advanced spectrometer operation topics such as direct- versus indirect-detection experiments and probe tuning. You will complete a hands-on, practical exercise during the training session. Miscellaneous Training Topics Additional NMR training courses such as for variable-temperature (VT) experiments, CapNMR probe use, solvent suppression methods, diffusion, relaxation or kinetics experiments are typically organized on an ad hoc basis. Please use the online NMR Training Registration Form to request or suggest additional training courses of interest.