{"id":173,"date":"2025-05-27T19:00:13","date_gmt":"2025-05-27T19:00:13","guid":{"rendered":"https:\/\/wwwtest.pharmacy.wisc.edu\/faculty\/kwon-research-group\/?p=173"},"modified":"2025-05-27T19:05:00","modified_gmt":"2025-05-27T19:05:00","slug":"production-of-paclitaxel-loaded-peg-b-pla-micelles-using-peg-for-drug-loading-and-freeze-drying","status":"publish","type":"post","link":"https:\/\/pharmacy.wisc.edu\/faculty\/kwon-research-group\/2025\/05\/27\/production-of-paclitaxel-loaded-peg-b-pla-micelles-using-peg-for-drug-loading-and-freeze-drying\/","title":{"rendered":"Production of paclitaxel-loaded PEG-b-PLA micelles using PEG for drug loading and freeze-drying"},"content":{"rendered":"
\r\n
October 1, 2022<\/div>\r\nCite this<\/a>\r\n\r\n<\/div>\r\n
\r\n
\r\n
\r\n

Abstract<\/h2>\r\n
\"Cover
https:\/\/doi.org\/10.1016\/j.jconrel.2022.08.032<\/figcaption><\/figure>\r\n\r\nA new approach named PEG-assist is introduced for the production of drug-loaded polymeric\u00a0micelles<\/a>. The method is based on the use of\u00a0PEG<\/a>\u00a0as the non-selective solvent for PEG-b<\/em>-PLA in the fabrication procedure. Both hydration temperature and PEG molecular weight are shown to have a significant effect on the encapsulation efficiency of\u00a0PTX<\/a>\u00a0in PEG4kDa<\/sub>\u2013b<\/em>-PLA2kDa<\/sub>\u00a0micelles. The optimal procedure for fabrication includes the use of PEG1kDa<\/sub>\u00a0as the solvent at 60\u00a0\u00b0C, cooling the mixture to 40\u00a0\u00b0C, hydration at 40\u00a0\u00b0C, freezing at \u221280\u00a0\u00b0C and freeze-drying at \u221235\u00a0\u00b0C, 15\u00a0Pa. No significant difference (p<\/em>\u00a0>\u00a00.05) in PTX encapsulation, average particle size and polydispersity index is observed between the samples before freeze-drying and after reconstitution of the freeze-dried cake. The prepared PTX formulations are stable at room temperature for at least 8\u00a0h. Scaling the batch size to 25\u00d7 leads to no significant change (p<\/em>\u00a0>\u00a00.05) in PTX encapsulation, average particle size and polydispersity index. PEG-assist method is applicable to other drugs such as 17-AAG, and\u00a0copolymers<\/a>\u00a0of varied molecular weights. The use of no\u00a0organic solvent<\/a>, simplicity, cost-effectiveness, and efficiency makes PEG-assist a very promising approach for large scale production of drug-loaded polymeric micelles.\r\n

Cited by<\/h2>\r\nThis article is cited by 7 publications\r\n
    \r\n \t
  1. \r\n
    Losada-Barreiro, S., Celik, S., Sezgin-Bayindir, Z., Bravo-Fern\u00e1ndez, S., & Bravo-D\u00edaz, C. (2024). Carrier Systems for Advanced Drug Delivery: Improving Drug Solubility\/Bioavailability and Administration Routes.\u00a0Pharmaceutics<\/i>,\u00a016<\/i>(7), 852.\u00a0https:\/\/doi.org\/10.3390\/pharmaceutics16070852<\/a><\/div><\/li>\r\n \t
  2. \r\n
    Shabatina, T. I., Gromova, Y. A., Vernaya, O. I., Soloviev, A. V., Shabatin, A. V., Morosov, Y. N., Astashova, I. V., & Melnikov, M. Y. (2024). Pharmaceutical Nanoparticles Formation and Their Physico-Chemical and Biomedical Properties.\u00a0Pharmaceuticals (Basel, Switzerland)<\/i>,\u00a017<\/i>(5), 587.\u00a0https:\/\/doi.org\/10.3390\/ph17050587<\/a><\/div><\/li>\r\n \t
  3. \r\n
    Mohammadzadeh, M., Zarei, M., Abbasi, H., Webster, T. J., & Beheshtizadeh, N. (2024). Promoting osteogenesis and bone regeneration employing icariin-loaded nanoplatforms.\u00a0Journal of biological engineering<\/i>,\u00a018<\/i>(1), 29.\u00a0https:\/\/doi.org\/10.1186\/s13036-024-00425-4<\/a><\/div><\/li>\r\n \t
  4. \r\n
    Rasoulianboroujeni, M., de Villiers, M. M., & Kwon, G. S. (2023). Entropy-Driven Liquid-Liquid Phase Separation Transition to Polymeric Micelles.\u00a0The journal of physical chemistry. B<\/i>,\u00a0127<\/i>(37), 7925\u20137936.\u00a0https:\/\/doi.org\/10.1021\/acs.jpcb.3c03854<\/a><\/div><\/li>\r\n \t
  5. \r\n
    Tiwari, H., Rai, N., Singh, S., Gupta, P., Verma, A., Singh, A. K., Kajal, Salvi, P., Singh, S. K., & Gautam, V. (2023). Recent Advances in Nanomaterials-Based Targeted Drug Delivery for Preclinical Cancer Diagnosis and Therapeutics.\u00a0Bioengineering (Basel, Switzerland)<\/i>,\u00a010<\/i>(7), 760.\u00a0https:\/\/doi.org\/10.3390\/bioengineering10070760<\/a><\/div><\/li>\r\n \t
  6. \r\n
    Vach Agocsova, S., Culenova, M., Birova, I., Omanikova, L., Moncmanova, B., Danisovic, L., Ziaran, S., Bakos, D., & Alexy, P. (2023). Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review.\u00a0Materials (Basel, Switzerland)<\/i>,\u00a016<\/i>(12), 4267.\u00a0https:\/\/doi.org\/10.3390\/ma16124267<\/a><\/div><\/li>\r\n \t
  7. \r\n
    Repp, L., Skoczen, S. L., Rasoulianboroujeni, M., Stern, S. T., & Kwon, G. S. (2023). Plasma Stability and Plasma Metabolite Concentration-Time Profiles of Oligo(Lactic Acid)8-Paclitaxel Prodrug Loaded Polymeric Micelles.\u00a0The AAPS journal<\/i>,\u00a025<\/i>(3), 39.\u00a0https:\/\/doi.org\/10.1208\/s12248-023-00807-4<\/a><\/div><\/li>\r\n<\/ol>\r\n<\/div>\r\n<\/div>\r\n<\/section><\/section><\/div>","protected":false},"excerpt":{"rendered":"October 1, 2022 Cite this Abstract A new approach named PEG-assist is introduced for the production of drug-loaded polymeric\u00a0micelles. The method is based on the use of\u00a0PEG\u00a0as the non-selective solvent for PEG-b-PLA in the fabrication …","protected":false},"author":7,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-173","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"acf":[],"_links":{"self":[{"href":"https:\/\/pharmacy.wisc.edu\/faculty\/kwon-research-group\/wp-json\/wp\/v2\/posts\/173","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pharmacy.wisc.edu\/faculty\/kwon-research-group\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pharmacy.wisc.edu\/faculty\/kwon-research-group\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pharmacy.wisc.edu\/faculty\/kwon-research-group\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/pharmacy.wisc.edu\/faculty\/kwon-research-group\/wp-json\/wp\/v2\/comments?post=173"}],"version-history":[{"count":2,"href":"https:\/\/pharmacy.wisc.edu\/faculty\/kwon-research-group\/wp-json\/wp\/v2\/posts\/173\/revisions"}],"predecessor-version":[{"id":178,"href":"https:\/\/pharmacy.wisc.edu\/faculty\/kwon-research-group\/wp-json\/wp\/v2\/posts\/173\/revisions\/178"}],"wp:attachment":[{"href":"https:\/\/pharmacy.wisc.edu\/faculty\/kwon-research-group\/wp-json\/wp\/v2\/media?parent=173"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pharmacy.wisc.edu\/faculty\/kwon-research-group\/wp-json\/wp\/v2\/categories?post=173"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pharmacy.wisc.edu\/faculty\/kwon-research-group\/wp-json\/wp\/v2\/tags?post=173"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}