{"id":313,"date":"2020-05-14T19:32:02","date_gmt":"2020-05-14T19:32:02","guid":{"rendered":"https:\/\/wwwtest.pharmacy.wisc.edu\/faculty\/hong-research-group\/?p=313"},"modified":"2025-06-20T19:34:54","modified_gmt":"2025-06-20T19:34:54","slug":"immunoavidity-based-capture-of-tumor-exosomes-using-polyamidoamine-dendrimer-surfaces","status":"publish","type":"post","link":"https:\/\/pharmacy.wisc.edu\/faculty\/hong-research-group\/2020\/05\/14\/immunoavidity-based-capture-of-tumor-exosomes-using-polyamidoamine-dendrimer-surfaces\/","title":{"rendered":"Immunoavidity-Based Capture of Tumor Exosomes Using Poly(amidoamine) Dendrimer Surfaces"},"content":{"rendered":"

Abstract<\/h2>\r\nTumor-derived blood-circulating\u00a0exosomes\u00a0have potential as a biomarker to greatly improve cancer treatment. However, effective isolation of\u00a0exosomes\u00a0remains a tremendous technical challenge. This study presents a novel nanostructured polymer\u00a0surface\u00a0for highly effective\u00a0capture\u00a0of\u00a0exosomes\u00a0through strong avidity.\r\n\r\nVarious\u00a0surface\u00a0configurations, consisting of multivalent\u00a0dendrimers, PEG, and\u00a0tumor-targeting antibodies, were tested\u00a0using\u00a0exosomes\u00a0isolated from\u00a0tumor\u00a0cell lines. We found that a dual layer\u00a0dendrimer\u00a0configuration exhibited the highest efficiency in\u00a0capturing\u00a0cultured\u00a0exosomes spiked into human serum. Importantly, the optimized surface\u00a0captured\u00a0a > 4-fold greater amount of\u00a0tumor\u00a0exosomes\u00a0from head and neck cancer patient plasma samples than that from healthy donors. Nanomechanical analysis\u00a0using\u00a0atomic force microscopy also revealed that the enhancement was attributed to multivalent binding (avidity) and augmented short-range adhesion mediated by\u00a0dendrimers. Our results support that the\u00a0dendrimer\u00a0surface\u00a0detects\u00a0tumor\u00a0exosomes\u00a0at high sensitivity and specificity, demonstrating its potential as a new cancer liquid biopsy platform.\r\n\r\n\"visualization\r\n\r\n
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Cited by<\/h2>\r\nThis article is cited by 16 publications\r\n
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    Poellmann, M. J., Bu, J., Liu, S., Wang, A. Z., Seyedin, S. N., Chandrasekharan, C., Hong, H., Kim, Y., Caster, J. M., & Hong, S. (2023). Nanotechnology and machine learning enable circulating tumor cells as a reliable biomarker for radiotherapy responses of gastrointestinal cancer patients.\u00a0Biosensors & bioelectronics<\/i>,\u00a0226<\/i>, 115117.\u00a0https:\/\/doi.org\/10.1016\/j.bios.2023.115117<\/a><\/div><\/li>\r\n \t
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    Yin, Y., Han, X., Li, C., Sun, T., Li, K., Liu, X., & Liu, M. (2022). The status of industrialization and development of exosomes as a drug delivery system: A review.\u00a0Frontiers in pharmacology<\/i>,\u00a013<\/i>, 961127.\u00a0https:\/\/doi.org\/10.3389\/fphar.2022.961127<\/a><\/div><\/li>\r\n \t
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    Poellmann, M. J., Rawding, P., Kim, D., Bu, J., Kim, Y., & Hong, S. (2022). Branched, dendritic, and hyperbranched polymers in liquid biopsy device design.\u00a0Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology<\/i>,\u00a014<\/i>(3), e1770.\u00a0https:\/\/doi.org\/10.1002\/wnan.1770<\/a><\/div><\/li>\r\n \t
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    Lee, T., Rawding, P. A., Bu, J., Hyun, S., Rou, W., Jeon, H., Kim, S., Lee, B., Kubiatowicz, L. J., Kim, D., Hong, S., & Eun, H. (2022). Machine-Learning-Based Clinical Biomarker Using Cell-Free DNA for Hepatocellular Carcinoma (HCC).\u00a0Cancers<\/i>,\u00a014<\/i>(9), 2061.\u00a0https:\/\/doi.org\/10.3390\/cancers14092061<\/a><\/div><\/li>\r\n \t
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    Nair, A., Bu, J., Rawding, P. A., Do, S. C., Li, H., & Hong, S. (2021). Cytochalasin B Treatment and Osmotic Pressure Enhance the Production of Extracellular Vesicles (EVs) with Improved Drug Loading Capacity.\u00a0Nanomaterials (Basel, Switzerland)<\/i>,\u00a012<\/i>(1), 3.\u00a0https:\/\/doi.org\/10.3390\/nano12010003<\/a><\/div><\/li>\r\n \t
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    Saad, M. G., Beyenal, H., & Dong, W. J. (2021). Exosomes as Powerful Engines in Cancer: Isolation, Characterization and Detection Techniques.\u00a0Biosensors<\/i>,\u00a011<\/i>(12), 518.\u00a0https:\/\/doi.org\/10.3390\/bios11120518<\/a><\/div><\/li>\r\n \t
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    Min, L., Wang, B., Bao, H., Li, X., Zhao, L., Meng, J., & Wang, S. (2021). Advanced Nanotechnologies for Extracellular Vesicle-Based Liquid Biopsy.\u00a0Advanced science (Weinheim, Baden-Wurttemberg, Germany)<\/i>,\u00a08<\/i>(20), e2102789.\u00a0https:\/\/doi.org\/10.1002\/advs.202102789<\/a><\/div><\/li>\r\n \t
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    Nair, A., Bu, J., Bugno, J., Rawding, P. A., Kubiatowicz, L. J., Jeong, W. J., & Hong, S. (2021). Size-Dependent Drug Loading, Gene Complexation, Cell Uptake, and Transfection of a Novel Dendron-Lipid Nanoparticle for Drug\/Gene Co-delivery.\u00a0Biomacromolecules<\/i>,\u00a022<\/i>(9), 3746\u20133755.\u00a0https:\/\/doi.org\/10.1021\/acs.biomac.1c00541<\/a><\/div><\/li>\r\n \t
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    Cheng, S., Li, Y., Yan, H., Wen, Y., Zhou, X., Friedman, L., & Zeng, Y. (2021). Advances in microfluidic extracellular vesicle analysis for cancer diagnostics.\u00a0Lab on a chip<\/i>,\u00a021<\/i>(17), 3219\u20133243.\u00a0https:\/\/doi.org\/10.1039\/d1lc00443c<\/a><\/div><\/li>\r\n \t
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    Bu, J., Lee, T. H., Poellmann, M. J., Rawding, P. A., Jeong, W. J., Hong, R. S., Hyun, S. H., Eun, H. S., & Hong, S. (2021). Tri-modal liquid biopsy: Combinational analysis of circulating tumor cells, exosomes, and cell-free DNA using machine learning algorithm.\u00a0Clinical and translational medicine<\/i>,\u00a011<\/i>(8), e499.\u00a0https:\/\/doi.org\/10.1002\/ctm2.499<\/a><\/div><\/li>\r\n \t
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    Herrmann, M., Diederichs, S., Melnik, S., Riegger, J., Trivanovi\u0107, D., Li, S., Jenei-Lanzl, Z., Brenner, R. E., Huber-Lang, M., Zaucke, F., Schildberg, F. A., & Gr\u00e4ssel, S. (2021). Extracellular Vesicles in Musculoskeletal Pathologies and Regeneration.\u00a0Frontiers in bioengineering and biotechnology<\/i>,\u00a08<\/i>, 624096.\u00a0https:\/\/doi.org\/10.3389\/fbioe.2020.624096<\/a><\/div><\/li>\r\n \t
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    Bu, J., Lee, T. H., Jeong, W. J., Poellmann, M. J., Mudd, K., Eun, H. S., Liu, E. W., Hong, S., & Hyun, S. H. (2020). Enhanced detection of cell-free DNA (cfDNA) enables its use as a reliable biomarker for diagnosis and prognosis of gastric cancer.\u00a0PloS one<\/i>,\u00a015<\/i>(12), e0242145.\u00a0https:\/\/doi.org\/10.1371\/journal.pone.0242145<\/a><\/div><\/li>\r\n \t
  16. \r\n
    Bu, J., Nair, A., Iida, M., Jeong, W. J., Poellmann, M. J., Mudd, K., Kubiatowicz, L. J., Liu, E. W., Wheeler, D. L., & Hong, S. (2020). An Avidity-Based PD-L1 Antagonist Using Nanoparticle-Antibody Conjugates for Enhanced Immunotherapy.\u00a0Nano letters<\/i>,\u00a020<\/i>(7), 4901\u20134909.\u00a0https:\/\/doi.org\/10.1021\/acs.nanolett.0c00953<\/a><\/div><\/li>\r\n<\/ol>","protected":false},"excerpt":{"rendered":"Abstract Tumor-derived blood-circulating\u00a0exosomes\u00a0have potential as a biomarker to greatly improve cancer treatment. However, effective isolation of\u00a0exosomes\u00a0remains a tremendous technical challenge. This study presents a novel nanostructured polymer\u00a0surface\u00a0for highly effective\u00a0capture\u00a0of\u00a0exosomes\u00a0through strong avidity. Various\u00a0surface\u00a0configurations, consisting of multivalent\u00a0dendrimers, …","protected":false},"author":7,"featured_media":315,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[4],"tags":[],"class_list":["post-313","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publications"],"acf":[],"_links":{"self":[{"href":"https:\/\/pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/posts\/313","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/comments?post=313"}],"version-history":[{"count":1,"href":"https:\/\/pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/posts\/313\/revisions"}],"predecessor-version":[{"id":316,"href":"https:\/\/pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/posts\/313\/revisions\/316"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/media\/315"}],"wp:attachment":[{"href":"https:\/\/pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/media?parent=313"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/categories?post=313"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/tags?post=313"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}