Seminars in Pharmaceutical Sciences

Ph.D. Thesis Defense

Qing Yu, Pharmaceutical Sciences, UW-Madison, (under the supervision of Prof. Lingjun Li)

Advancing Qualitative and Quantitative Proteomics/Peptidomics via Development of Novel Mass Spectrometry-based Approaches

Abstract:  Liquid chromatography mass spectrometry (LC-MS) has evolved as a powerful tool in protein and peptide identification and quantitation.  It has the capability to monitor thousands of proteins and peptides simultaneously and therefore is a critical component in the study of underlying biological mechanisms and the discovery of novel therapeutic targets.  This dissertation is devoted to the development and application of novel LC-MS based techniques to characterize proteomics and peptidomics qualitatively and quantitatively by incorporating various sample preparation strategies, novel MS methods and isobaric labeling technology.

     This dissertation research not only improves upon isobaric labeling technique by exploring novel chemical tags and new fragmentation methods for quantitative proteomics, but also presents a useful platform to enable in-depth investigation on complex protein post-translational modifications (PTMs), such as phosphorylation and glycosylation.

Monday, April 10, 2017, 2:00 p.m., 1227 Cooper Hall (School of Nursing)